First, due to the numerous subtypes of hepatitis B virus, the primer sequence can only identify some of these subtypes, but not all of them. The HBV subtypes that do not match the primer-binding regions cannot be detected.
To some extent, it limits the wide usage of this method. Second, DNA extraction process is still required. Although some reagent, such as chelex, has simplified the DNA extraction process, the DNA extraction process is still a limitation of the entire process.
Our method did not combine the DNA extraction and rapid amplification into one step. This fast and easy-to-read-out method has the potential application in the developing countries with low-resource laboratories. The studies involving human participants were reviewed and approved by the Ethics Committee of China Medical University.
M-LS and JY designed and drafted the work and wrote the manuscript. Z-YZ and BP performed sample preparation. JY conceived and interpreted experiments. The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers.
Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher. National Center for Biotechnology Information , U.
Journal List Front Mol Biosci v. Front Mol Biosci. Published online Dec 2. Author information Article notes Copyright and License information Disclaimer. This article was submitted to Molecular Diagnostics and Therapeutics, a section of the journal Frontiers in Molecular Biosciences.
Received Aug 23; Accepted Nov 8. The use, distribution or reproduction in other forums is permitted, provided the original author s and the copyright owner s are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Abstract Hepatitis B virus infection is not only a huge burden in the field of social health but also a major public health problem that affects the lives and health of the people. Keywords: hepatitis B virus, multienzyme isothermal rapid amplification, lateral flow strip, detection, nucleic acid. Open in a separate window. CPT Code s Ordering Restrictions may apply. Test Resources. Service Area must be determined. Room temperature: Unacceptable Refrigerated: 6 days Frozen: 84 days.
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